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Chia-Lin Chyan

Professor,  Biochemistry





Lab    :(03)-890-3590


D421 Science building 


D416、D417 Science building 



  Personal Website



1980-1984: National Tsing Hua University department of chemistry

1988-1994: Rutgers University department of chemistry Ph.D.



1988-1991: Rutgers University TA
1991-1994: Rutgers University RA
1994-1999: Academia sinica Postdoctoral research fellow
1999-2005.08: NDHU Assistant professor of department of chemistry
2005.08-2017.08: NDHU Associate professor of department of chemistry
2017.08-now: NDHU professor of department of chemistry

  Research Interests


1. Biochemistry
2. Nuclear magnetic resonance in structural biology
3. Biophysical chemistry
4. Structural biology 

  Selected Publications


1. Y.-L. Yeh, C.-H. Chang, C.-L. Chyan, and S.-H. Lin, “Atomic Force Microscopic and Theoretical Study of Protein Folding and Unfolding”, Instruments Today 25 (5), 39-45 (2004)

2. C.-M. Chen, C.-L. Chyan, T.-T. Lee, S.-H. Huang, and J. T.C. Tzen, “Caleosin Stabilizes Artificial Oil Bodies as Nanoparticles”, J. of Agri. And Food Chem. 52 (12), 3982-7 (2004) (SCI).

3. T.-H. Lin, J.-W.Huang, H..-b.. Huang, Y..-c.. Chen, C.-Y. Liu, C.-j. Lo, T. C. Tang, and C.-L. Chyan, “1H, 15N, and 13C Resonance Assignments of Calmodulin Complexed with the Calmodulin-Binding Domain of Protein Phosphatase 2B”, J. of Biomol. NMR, 29, 531-2 (2004) (SCI).

4. J.-H. Shyu, C.-L. Chyan, J. T. C. Tzen, and W.-M. Chou, “Molecular Cloning, Expression, and Functional Characterization of a Cystatin from Pineapple Stem”, Bioscience, Biotechnology, and Biochemistry 68 (8), 1681-9 (2004) (SCI).

5. C.-L. Chyan, F.-C. Lin, H. Peng, J.-M. Yuan, C.-H. Chang, S.-H. Lin, and G. Yang, “Reversible Mechanical Unfolding of Single Ubiquitin Molecules”, Biophysical J. 87, 3995-4006(2004) (SCI).

6. Y.-L. Yeh, C.-H. Chang, K.-K. Liang, Y.-J. Shiu, C. Su, M. Hayashi, C.-L. Chyan, G. Yang, Y. Moe, Y.-J. Yan, S.-H. Lin, “Atomic force microscopic and theoretical studies of poly-ubiquitin proteins”, Chemical Physics Letters 399, 440–445 (2004) (SCI).

7. C.-L. Chyan, P.-C. Huang, T.-H. Lin, J.-W. Huang, S.-S. Lin, H.-b. Huang and Y.-C. Chen, “Purification, crystallization and preliminary crystallographic studies of the Camodulin-OLFp complex” Acta Crystallographica F61, 785-787 (2005) (SCI).

8. C.-L. Chyan, T.T.-T. Lee, C.-P. Liu, Y.-C. Yang, J.T.-C. Tzen, and W.-M. Chou, “Cloning and Expression of a Seed-Specific Metallothionein-Like Protein from Sesame”Bioscience, Biotechnology, and Biochemistry 69 (12), 344-351 (2005) (SCI).

9. Y.R. Chen, H.B. Huang, C.-L. Chyan, M.S. Shiao, T.H. Lin and Y.C. Chen, “The Effect of A Conformation on the Metal Affinity and Aggregation Mechanism Studied by Circular  Dichroism Spectroscopy”, J. Biochem. 139, 733–740 (2006) (SCI).

10. M.Q. Liao, Y.J. Tzeng, Lea Y.X. Chang, H.B. Huang, T.H. Lin, C.L. Chyan, Y.C.Chen,* “The Correlation between Neurotoxicity, Aggregative Ability and Secondary 2 Structure Studied by Sequence Truncated A peptides” FEBS Lett 581, 1161-1165. (2007) (SCI IF 3.264)

11. A. C. N. Chua, W.-M. Chou, C.-L. Chyan, J. T. C. Tzen*, "Purification, cloning, and identification of two thaumatin-like protein lsoforms in jelly fig (Ficus awkeotsang) achenes" J. of Agri. Food Chem 55 (18), 7602-7608 (2007) (SCI IF 2.562)

12. Y. M.Yuan, C.-L.Chyan, H. X. Zhou, T. Y. Chung, H. Peng, G. Ping, G. Yang*,

"The effects of macromolecular crowding on the mechanical stability of protein molecules" Protein Sci. 17(12), 2156-66 (2008) (SCI IF 3.115).

13. Liu, T. H., Chyan, C. L., Li, F. Y., and Tzen, J. T. (2009) Stability of artificial oil bodies constituted with recombinant caleosins, J Agric Food Chem 57, 2308-2313 (2009 March).

14. Huang, Y. C., Chen, Y. C., Tsay, H. J., Chyan, C. L., Chen, C. Y., Huang, H. B., and Lin, T. H. (2010) The effect of PKA-phosphorylation on the structure of inhibitor-1 studied by NMR spectroscopy, J Biochem 147, 273-278 (2010 Feb).

15. Lu, H. C., Jiang, P. L., Hsu, L. R., Chyan, C. L., and Tzen, J. T. (2010)

Characterization of oil bodies in adlay (Coix lachryma-jobi L), Biosci Biotechnol Biochem 74, 1841-1847 (2010 Sep).

16. Liu, T. H., Chyan, C. L., Li, F. Y., Chen, Y. J., and Tzen, J. T. C. (2011) Engineering lysine-rich caleosins as carrier proteins to render biotin as a hapten on artificial oil bodies for antibody production, Biotechnol Progr 27, 1760-1767 (2011 Nov).

17. Irene, D., Chen, B. J., Lo, S. H., Liu, T. H., Tzen, J. T., and Chyan, C. L.(2012) Resonance assignments and secondary structure of a phytocystatin from Ananas comosus, Biomol NMR Assign 6, 99-101 (2012 April).

18. Zulueta, M. M., Lin, S. Y., Lin, Y. T., Huang, C. J., Wang, C. C., Ku, C. C., Shi, Z., Chyan, C. L., Irene, D., Lim, L. H., Tsai, T. I., Hu, Y. P., Arco, S. D., Wong, C. H., and Hung, S. C. (2012) alpha-Glycosylation by D-glucosamine-derived donors: synthesis of heparosan and heparin analogues that interact with mycobacterial heparin-binding hemagglutinin, J Am Chem Soc 134, 8988-8995 (2012 May).

19. Chen, D. H., Chyan, C. L., Jiang, P. L., Chen, C. S., and Tzen, J. T. (2012) The same oleosin isoforms are present in oil bodies of rice embryo and aleurone layer while caleosin exists only in those of the embryo, Plant Physiol Biochem 60C, 18-24 (2012 Aug epub).

20. Irene, D., Huang, J. W., Chung, T. Y., Li, F. Y., Tzen, J. T., Lin, T. H., and Chyan, C. L. (2012) Binding orientation and specificity of calmodulin to rat olfactory cyclic nucleotide-gated ion channel, J Biomol Struct Dyn. (2012 Aug epub)

21. Jheng, Y. J., Tsai, W. Y., Chen, K. H., Lin, K. W., Chyan, C. L., Yang, C. C., and Lin, K. C. (2012) Recombinant dioscorins of the yam storage protein expressed in Escherichia coli exhibit antioxidant and immunomodulatory activities, Protein Expr Purif 85, 77-85 (2012 Sep epub).